Jyothi B. Nair

Tracking the Foot Prints of Bio-molecules via SERS Trajectory
Council of Scientific and Industrial Research // Thiruvananthapuram, India

Q&A Session III // Friday, October 30 // 8.15 am – 9 am (CET)

 The implementation of Raman and surface enhanced Raman spectroscopy (SERS) for the detection of disease has increased tremendous attention over the recent decades. SERS is a highly sensitive and non-destructive technique, which enable to provide unique fingerprint vibrational spectra of surface species with high sensitivity, even up to single-cell and single-molecular level. This excellent platform can be used for visualising the internalisation and monitoring the therapeutic effects especially the mechanistic events is a challenging task. In the present work, synthesis of spherical and hollow gold nano particle based theranostic probe development and its utilisation for revealing the biomolecular events is demonstrated. The as prepared spherical gold nanoprobe is utilised for tracking the internalisation as well as microtubule disruption events of non fluorescent drug paclitaxel (PTX). SERS fingerprinting and imaging for visualising the effect of the targeted theranostic platform with novel hollow gold nanoparticles for monitoring the molecular level therapeutic progression of combination therapy in cells, spheroidal model is also verified. The promising findings and the analytical validation indicate which steps are to be taken on the way toward a clinical validation. 

References: 

1) Kang, B.; Austin, L. A.; El-Sayed, M. A. Observing Real- Time Molecular Event Dynamics of Apoptosis in Living Cancer Cells Using Nuclear-Targeted Plasmonically Enhanced Raman Nanoprobes. ACS Nano 2014, 8 (5), 4883.4892. 

2) Sujai, P. T.; Joseph, M. M.; Saranya, G.; Nair, J. B.; Murali, V. P.; Maiti, K. K. Surface Charge Modulates the Internalization vs Penetration of Gold Nanoparticles: A Comprehensive Scrutiny on Monolayer Cancer Cells, Multicellular Spheroids and Solid Tumor by SERS Modality. Nano scale 2020.12, 6971-6975. 

3) Joseph, M. M.; Ramya, A. N.; Vijayan, V. M.; Nair, J. B.; Bastian, B. T.; Pillai, R. K.; Therakathinal, S. T.; Maiti, K. K. Targeted Theranostic Nano Vehicle Endorsed with Self- Destruction and Immunostimulatory Features to Circumvent Drug Resistance and Wipe-Out Tumor Reinitiating Cancer Stem Cells. Small 2020.DOI: 10.1002/smll.202003309. 

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